The ABI 3130xl system uses capillary electrophoresis which is significantly advantageous over slab-gel based technology. Instead of risking inconsistency as a result of pouring slab-gels, a simple capillary array is fit into the available slots. Moreover, the arrays are guaranteed for 100 runs (rather than one slab per run) and there is no sample loss, unlike a slab-gel. The fragments of DNA (and all other negatively charged molecules) are attracted to the platinum cathode electrode attached to the end of the capillary (16 capillaries in all), which is open and filled with Performance Optimized Polymer (POP).When current is applied, the DNA fragments are attracted to the capillary and travel through the polymer to the detection window and array housing. As the fragments pass through a clear area in the capillary, the fluorophores are excited by a laser and emission is detected and recorded by a CCD camera (the 3130 series can detect up to five fluorophores). The ability to detect multiple fluorophores enables detection of a larger number of similar size DNA fragments.
Key Features -
• 16 Capillaries
• 24 Hour unattended operation
• Detection cell heated for improved thermal control
• Automated sample injection from both 96 and 384 well microplates
• POP-7, POP-6 & POP-4 separation polymers
Includes - ABI-3130xl (16 capillary), Sapphire Pump, Pentium IV Data Station (400 GB hard drive), Software Pre-loaded, Buffer Anode Jar, Buffer / Water / Waste reservoirs with lids and service report.
For more information please contact Joe@harlowscientific.com
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